To fire Lightbox V3 on page load, add the following code to the page. Do this after the Lightbox Setup layout.
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<script type="text/javascript">$(document).ready(function(){$('[rel^="lightbox"]:first').click(); });</script>
If you want this to be on one / some pages only, you could put it into a Fragment Text or Product Deescription like:
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!!<<script type="text/javascript">$(document).ready(function(){$('[rel^="lightbox"]:first').click(); });</script>>!!
What this does is looks for the first Lightbox link on the page and fires it's click event.